Silylation reagents - BSTFA, SILYL-991
For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation.
Reagents for silylation - alkylation (methylation) - acylation are available.
N,O-bis-trimethylsilyl-trifluoroacetamide
m.w. 257.4, Bp 40°C (12mm Hg), density d20°/4° = 0.961
BSTFA: R = CF3 powerful trimethylsilyl donor with approximately the same donor strength as the non-fluorinated analogue BSA
advantage of BSTFA over BSA: greater volatility of its reaction products (particularly useful for GC of some lower boiling TMS amino acids.

BSTFA is nonpolar (less polar than MSTFA), and can be mixed with acetonitrile for improved solubility. For silylating fatty acid amides, hindered hydroxyls and other compounds, which are difficult to silylate (like secondary alcohols and amines), we recommend BSTFA + 1% trimethylchlorosilane (TMCS), available under the designation SILYL-991.